DNA methylation is emerging as an attractive marker that can provide investigative leads to solve crimes in forensic genetics. Identification of body fluids that utilizes tissue-specific DNA methylation can contribute to solving crimes by allowing predicting activity that led to the deposition of the evidence material. DNA methylation-based age estimation is expected to be useful in helping to reduce the number of potential suspects, when the DNA profile from the evidence does not match that of any known person, including those stored in the forensic database. In addition, DNA methylation variation implicates environmental exposure, such as cigarette smoking and alcohol consumption, thereby suggesting the possibility to be used as a marker for predicting the lifestyle of potential suspect. This review will describe recent advances in our understanding of DNA methylation variations and discuss the utility of DNA methylation as a forensic marker to get more investigative leads from evidence materials.