| Mycobacterium abscessus D-alanyl-D-alanine dipeptidase induces the maturation of dendritic cells and promotes Th1-biased immunity |
| Seung Jun Lee1, Jong-Hwa Jang2, Gun Young Yun1, Da Rae Kang1, Hee Jo Park1, Sung Jae Shin3, Hee Dong Han1, Tae Heung Kang1, Won Sun Park4, Young Kyung Yoon5, In Duk Jung1, Yeong-Min Park1,* |
1Department of Immunology, Konkuk University,
2Department of Dental Hygiene, Hanseo University,
3Department of Microbiology, Yonsei University College of Medicine,
4Department of Physiology, Kangwon National University,
5Division of Infectious Diseases, Korea University |
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Abstract
Mycobacterium abscessus, a member of the group of non-tuberculous mycobacteria, has been identified as an emerging pulmonary pathogen in humans. However, little is known about the protective immune response of antigen-presenting cells, such as dendritic cells (DCs), which guard against M. abscessus infection. The M. abscessus gene MAB1843 encodes D-alanyl-D-alanine dipeptidase, which catalyzes the hydrolysis of D-alanyl-D-alanine dipeptide. We investigated whether MAB1843 is able to interact with DCs to enhance the effectiveness of the host’s immune response. MAB1843 was found to induce DC maturation via toll-like receptor 4 and its downstream signaling pathways, such as the mitogen-activated protein kinase and nuclear factor kappa B pathways. In addition, MAB1843-treated DCs stimulated the proliferation of T cells and promoted Th1 polarization. Our results indicate that MAB1843 could potentially regulate the immune response to M. abscessus, making it important in the development of an effective vaccine against this mycobacterium.
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| Abstract, Accepted Manuscript(in press) [Submitted on May 10, 2016, Accepted on July 19, 2016] |
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