| Promotion of formyl peptide receptor 1-mediated neutrophil chemotactic migration by antimicrobial peptides isolated from the centipede Scolopendra subspinipes mutilans |
| Yoo Jung Park1, Sung Kyun Lee1, Young Su Jung1, Mingyu Lee2, Ha Young Lee1, Sang Doo Kim1, Joon Seong Park3, JaeHyung Koo4, Jae Sam Hwang5, Yoe-Sik Bae1,2,* |
1Biological Sciences and 2Health Sciences and Technology, Sungkyunkwan University,
3Hematology-Oncology, Ajou University School of Medicine,
4Brain and Cognitive Sciences, DGIST,
5Agricultural Biology, National Academy of Agricultural Science |
|
Abstract
We investigated the effects of two antimicrobial peptides (AMPs) isolated from Scolopendra subspinipes mutilans on neutrophil activity. Stimulation of mouse neutrophils with the two AMPs elicited chemotactic migration of the cells in a pertussis toxin-sensitive manner. The two AMPs also stimulated activation of ERK and Akt, which contribute to chemotactic migration of neutrophils. We found that AMP-stimulated neutrophil chemotaxis was blocked by a formyl peptide receptor 1 (FPR1) antagonist (cyclosporin H); moreover the two AMPs stimulated the chemotactic migration of FPR1-expressing RBL-2H3 cells but not of vector-expressing RBL-2H3 cells. We also found that the two AMPs stimulate neutrophil migration in vivo, and that this effect is blocked in FPR1-deficient mice. Taken together, our results suggest that the two AMPs stimulate neutrophils, leading to chemotactic migration through FPR1, and the two AMPs will be useful for the study of FPR1 signaling and neutrophil activation.
|
| Abstract, Accepted Manuscript(in press) [Submitted on June 14, 2016, Accepted on August 8, 2016] |
|