BMB Reports Papers in Press available online.

Search Papers In Press
This galley proof is being listed electronically before publishing the final manuscript (It's not final version).

 
Caffeine-induced food-avoidance behavior is mediated by neuroendocrine signals in Caenorhabditis elegans
Hyemin Min1,#, Esther Youn1,#, Ichiro Kawasaki1, Yhong-Hee Shim1,*
1Bioscience and Biotechnology, Konkuk University
Abstract
High-dose caffeine uptake is a developmental stressor and causes food-avoidance behavior (aversion phenotype) in C. elegans, but its mode of action is largely unknown. In this study, we investigated the molecular basis of the caffeine-induced aversion behavior in C. elegans. We found that aversion phenotype induced by 30 mM caffeine was mediated by JNK/MAPK pathway, serotonergic and dopaminergic neuroendocrine signals. In this process, the dopaminergic signaling appears to be the major pathway because the reduced aversion behavior in cat-2 mutants and mutants of JNK/MAPK pathway genes was significantly recovered by pretreatment with dopamine. RNAi depletion of hsp-16.2, a cytosolic chaperone, and cyp-35A family reduced the aversion phenotype, which was further reduced in cat-2 mutants, suggesting that dopaminergic signal is indeed dominantly required for the caffeine-induced food aversion. Our findings suggest that aversion behavior is a defense mechanism for worms to survive under the high-dose caffeine conditions.
Abstract, Accepted Manuscript(in press) [Submitted on July 26, 2016, Accepted on September 29, 2016]
  Copyright © KSBMB. All rights reserved. / Powered by INFOrang.co., Ltd