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This galley proof is being listed electronically before publishing the final manuscript (It's not final version).

 
CRISPR as a strong gene editing tool
shengfu shen1, tiing jen loh1, hongling shen1, xuexiu zheng1, haihong Shen1,*
1Willston Northampton School,
2life siences, gwangju institute of science and technology
Abstract
Clustered regularly-interspaced short palindromic repeats (CRISPR) is a new and effective genetic editing tool. CRISPR was initially found in bacteria to protect it from viruse invasions. In the first step, specific DNA strands of virus are identified by guide RNA that is composed of crRNA and tracrRNA. Then RNAse III is required for producing crRNA from pre-crRNA. In The second step, a crRNA:tracrRNA:Cas9 complex guides RNase III to cleave target DNA. After cleavage of DNA by CRISPR-Cas9, DNA can be fixed by Non-Homologous End Joining (NHEJ) and Homology Directed Repair (HDR). Whereas NHEJ is simple and random, HDR is much more complex and accurate. Gene editing by CRIPR is able to be applied to various biological field such as agriculture and treating genetic diseases in human.
Abstract, Accepted Manuscript(in press) [Submitted on July 28, 2016, Accepted on September 7, 2016]
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