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Expression and characterization of transmembrane and coiled-coil domain family 3
Wern-Joo Sohn1, Jae-Young Kim1, Dongbum Kim2, Jeong-A Park3, Younghee Lee3, Hyung-Joo Kwon2,4,*
1Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University,
2Center for Medical Science Research, College of Medicine, Hallym University,
3Department of Biochemistry, College of Natural Sciences, Chungbuk National University,
4Department of Microbiology, College of Medicine, Hallym University
Transmembrane and coiled-coil domain family 3 (TMCC3) has been reported to be expressed in the human brain; however, its function is still unknown. Here, we found that expression of TMCC3 is higher in human whole brain, testis, and spinal cord compared to other human tissues. TMCC3 was expressed in mouse developing hind brain, lung, kidney, and somites, with strongest expression in the mesenchyme of developing tongue. By expression of recombinant TMCC3 and its deletion mutants, we found that TMCC3 proteins self-assemble to oligomerize. Immunostaining and confocal microscopy data revealed that TMCC3 proteins are localized in endoplasmic reticulum through transmembrane domains. Based on immunoprecipitation and mass spectroscopy data, TMCC3 proteins associate with TMCC3 and 14-3-3 proteins. This supports the idea that TMCC3 proteins form oligomers and that 14-3-3 may be involved in the function of TMCC3. Taken together, these results may be useful for better understanding of uncharacterized function of TMCC3.
Abstract, Accepted Manuscript(in press) [Submitted on August 29, 2016, Accepted on September 27, 2016]
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