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This galley proof is being listed electronically before publishing the final manuscript (It's not final version).

Extracellular vesicles as novel carrier for therapeutic molecules
Nambin Yim1, Chulhee Choi1,2,3,*
1Department of Bio and Brain Engineering, KAIST, Daejeon 34141 Korea,
2Cellex Life Sciences Inc., Daejeon, 34141 Korea,
3Cancer Metastasis Control Center, KAIST Institute for the Biocentury, KAIST, Daejeon 34141 Korea
Extracellular vesicles (EVs) are natural carriers of biomolecules that play central roles in cell-to-cell communications. Based on this, there have been many attempts to use EVs as therapeutic drug carriers. Various macromolecules, from chemical reagents to nucleic acids, were successfully loaded into EVs; however, loading of proteins with high molecular weight has been huddled with several problems. Purification of recombinant proteins is expensive and time consuming, and easily results in modification of proteins by physical or chemical forces. And the loading efficiency of conventional methods is too low for most of proteins. We have recently proposed a new method so-called exosomes for protein loading via optically reversible protein-protein interaction (EXPLORs) to overcome the limitations. Because EXPLORs are produced by actively loading of intracellular proteins into EVs using blue light without protein purification steps, we demonstrated that EXPLOR technique significantly improves the loading and delivery efficiency of therapeutic proteins. We further showed the potential of EXPLOR technology as a novel platform of biopharmaceuticals by successful delivery of several functional proteins such as Cre recombinase into the target cells in vitro and in vivo.
Abstract, Accepted Manuscript(in press) [Submitted on October 10, 2016, Accepted on October 11, 2016]
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