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hnRNPK-regulated PTOV1-AS1 modulates heme oxygenase-1 expression via miR-1207-5p
Chang Hoon Shin1, Seongho Ryu2, Hyeon Ho Kim1,3,*
1Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences and Technology, Sungkyunkwan University,
2Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University,
3Institute for Future Medicine, Samsung Medical Center
Abstract
Antisense transcripts were initially identified as transcriptional noise, but have since been reported to play an important role in the quality control of miRNA functions. In this report, we tested the hypothesis that hnRNPK regulates miRNA function via competitive endogenous RNAs, such as pseudogenes, long non-coding RNAs, and antisense transcripts. Based on analyses of RNA sequencing data, the knockdown of hnRNPK decreased antisense PTOV1-AS1 transcript, which harbors five binding sites for miR-1207-5p. We identified HO-1 mRNA as a novel target of miR-1207-5p by western blotting and Ago2 immunoprecipitation. The knockdown of hnRNPK or PTOV1-AS1 suppressed heme oxygenase-1 (HO-1) expression by increasing the enrichment of HO-1 mRNA in miR-1207-5p-mediated miRISC. Downregulation of HO-1 by a miR-1207-5p mimic or knockdown of hnRNPK and PTOV1-AS1 inhibited proliferation and clonogenic ability. Taken together, our results demonstrate that hnRNPK-regulated PTOV1-AS1 modulates HO-1 expression via miR-1207-5p.
Abstract, Accepted Manuscript(in press) [Submitted on February 9, 2017, Accepted on February 22, 2017]
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