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Post-transcriptional and translational regulation of mRNA-like long non-coding RNAs by microRNAs in early developmental stages of zebrafish embryos
Kyung-Tae Lee1, Jin-Wu Nam1,*
1Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, Korea
Abstract
microRNA (miRNA) represses protein-coding gene via seed pairing to 3∏ UTRs of mRNA at the post-transcriptional and translational level. Although working models of miRNA-mediated gene silencing have been successfully established using miRNA transfections and knockouts, the regulatory interaction between miRNA and long non-coding RNA (lncRNA) remain unknown. In particular, how mRNA-resembling lncRNAs with 5’ cap, 3’ poly(A)-tail, or coding features are regulated by miRNA is yet to be examined. We therefore investigated the functional interaction between miRNAs and lncRNAs with/without those features in miRNA-transfected, early zebrafish embryos. We observed that the lncRNAs verified to contain 5’ cap, 3’ poly(A)-tail, and canonical miRNA target sites were observed to be repressed in the level of both RNA and ribosome-protected fragment, while those with miRNA target sites and without 5’ cap and 3; poly(A)-tail were not robustly repressed by miRNA introduction, suggesting a role as a miRNA-decoy.
Abstract, Accepted Manuscript(in press) [Submitted on February 11, 2017, Accepted on March 21, 2017]
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