| Heterogeneous interaction network of yeast prions and remodeling factors detected in live cells |
| Chan-Gi Pack1,*, Yuji Inoue2,3, Takashi Higurashi3, Shigeko Kawai-Noma2, Daigo Hayashi2, Elizabeth Craig3, Hideki Taguchi2 |
1Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine,
2Department of Biomolecular Engineering, Tokyo Institute of Technology,
3Department of Biochemistry, University of Wisconsin,
4Department of Virology, Osaka University |
|
Abstract
Budding yeast has at least dozens of prions, which are mutually dependent on each other for the de novo prion formation. In addition to the interactions among prions, transmissions of prions are strictly dependent on two chaperone systems: Hsp104 and the Hsp70/Hsp40 (J-protein) system, both of which cooperatively remodel the prion aggregates to ensure the multiplication of prion entities. Since it has been postulated that prions and the remodeling factors constitute complex networks in cells, a quantitative approach to describe the interactions in live cells would be required. Here, we applied the dual-color fluorescence cross-correlation spectroscopy to investigate the molecular network of interaction in single live cells. The result demonstrats that yeast prions and remodeling factors constitute a network each other through heterogenous protein-protein interactions.
|
| Abstract, Accepted Manuscript(in press) [Submitted on May 24, 2017, Accepted on August 31, 2017] |
|