SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion |
Heegyum Moon1, Sunghee Cho1, Tiing Jen Loh1, Ha Na Jang1, Yongchao Liu1, Namjeong Choi1, Jagyeong Oh1, Jiyeon Ha1, Jianhua Zhou2, Sungchan Cho3, Dong-Eun Kim4, Michael B. Ye5, Xuexiu Zheng1, Haihong Shen1,* |
1School of Life Sciences, Gwangju Institute of Science and Technology, 2JiangSu Key Laboratory of Neuroregeneration, Nantong University, 3Bio-Therapeutics Research Institute, Korea Research Institute of Bioscience and Biotechnology, 4Department of Bioscience and Biotechnology, Konkuk University, 5Division of Liberal Arts and Sciences, Gwangju Institute of Science and Technology |
Abstract
SRSF2, a Serine-Arginine rich (SR) protein, is a splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron splicing to suppress cassette exon inclusion in SMN pre-mRNA. Through a serial mutagenesis, we demonstrate that a 10 nt RNA sequence surrounding the branch-point (BP), is important for SRSF2-mediated inhibition of cassette exon inclusion through directly interacting with SRSF2. We conclude that SRSF2 inhibits intron splicing to promote exon exclusion.
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Abstract, Accepted Manuscript(in press) [Submitted on June 16, 2017, Accepted on July 17, 2017] |
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