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This galley proof is being listed electronically before publishing the final manuscript (It's not final version).

 
SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion
Heegyum Moon1, Sunghee Cho1, Tiing Jen Loh1, Ha Na Jang1, Yongchao Liu1, Namjeong Choi1, Jagyeong Oh1, Jiyeon Ha1, Jianhua Zhou2, Sungchan Cho3, Dong-Eun Kim4, Michael B. Ye5, Xuexiu Zheng1, Haihong Shen1,*
1School of Life Sciences, Gwangju Institute of Science and Technology,
2JiangSu Key Laboratory of Neuroregeneration, Nantong University,
3Bio-Therapeutics Research Institute, Korea Research Institute of Bioscience and Biotechnology,
4Department of Bioscience and Biotechnology, Konkuk University,
5Division of Liberal Arts and Sciences, Gwangju Institute of Science and Technology
Abstract
SRSF2, a Serine-Arginine rich (SR) protein, is a splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron splicing to suppress cassette exon inclusion in SMN pre-mRNA. Through a serial mutagenesis, we demonstrate that a 10 nt RNA sequence surrounding the branch-point (BP), is important for SRSF2-mediated inhibition of cassette exon inclusion through directly interacting with SRSF2. We conclude that SRSF2 inhibits intron splicing to promote exon exclusion.
Abstract, Accepted Manuscript(in press) [Submitted on June 16, 2017, Accepted on July 17, 2017]
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