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MiR-204 acts as a potential therapeutic target in acute myeloid leukemia by increasing BIRC6-mediated apoptosis
Zhiguo Wang1,2,#, Hong Luo3,#, Zehui Fang4, Yanling Fan2, Xiaojuan Liu2, Yujing Zhang4, Shuping Rui2, Yafeng Chen2, Luojia Hong4, Jincheng Gao4, Mei Zhang1,*
1Hematology, the First Affiliated Hospital of Xi’an Jiaotong University,
2Bone Marrow Transplantation, Harbin Hematological Cancer Institute,
3Hematology, the First Hospital of Qiqihar,
4Endocrinology, the 4th Affiliated Hospital of Harbin Medical University
Abstract
Acute myeloid leukemia (AML) is one of the most common hematological malignancies all around the world. MicroRNAs have been determined to contribute various cancers initiation and progression, including AML. Although microRNA-204 (miR-204) exerts anti-tumor effects in several kinds of cancers, its function in AML remains unknown. In the present study, we assessed miR-204 expression in AML blood samples and cell lines. We also investigated the effects of miR-204 on cellular function of AML cells and the underlying mechanisms of the action of miR-204. Our results showed that miR-204 expression was significantly downregulated in AML tissues and cell lines. In addition, overexpression of miR-204 induced growth inhibition and apoptosis in AML cells, including AML5, HL-60, Kasumi-1 and U937 cells. Cell cycle analysis further confirmed an augmentation in theapoptotic subG1 population by miR-204 overexpression. Mechanistically, baculoviral inhibition of apoptosis protein repeat containing 6 (BIRC6) was identified as a direct target of miR-204. Enforcing miR-204 expression increased the luciferase activity and expression of BIRC6, as well as p53 and Bax expression. Moreover, restoration of BIRC6 reversed the pro-apoptotic effects of miR-204 overexpression in AML cells. Taken together, this study demonstrates that miR-204 causes AML cell apoptosis by targeting BIRC6, suggesting miR-204 may play an anti-carcinogenic role in AML and function as a novel biomarker and therapeutic target for the treatment of this disease.
Abstract, Accepted Manuscript(in press) [Submitted on February 15, 2018, Accepted on April 23, 2018]
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