| Protective effects of Tat-DJ-1 protein against streptozotocin (STZ)-induced diabetes in a mice model |
| Soo Young Choi1,*, Hyeon Ji Yeo1, Eun Ji Yeo1, Min Jea Shin1, Yeon Joo Choi1, Chi Hern Lee1, Hyeok Yil Kwon1, Dae Won Kim1, Won Sik Eum1, Soo Young Choi1 |
1Biomedical Science and Research Institute of Bioscience and Biotechnology and 2Physiology, College of Medicine, Hallym University,
3Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung-Wonju National University |
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Abstract
A major feature of type 1 diabetes mellitus is hyperglycemia and dysfunction of pancreatic モ-cells. In a previous study, we showed that Tat-DJ-1 protein inhibits pancreatic RINm5F モ-cell death caused by oxidative stress. In this study, we examine the effects of Tat-DJ-1 protein on streptozotocin (STZ)-induced diabetic mice. Wild type (WT) Tat-DJ-1 protein transduced into pancreas where it markedly inhibited pancreatic モ-cell destruction and regulated the level of serum parameters including insulin, alkaline phosphatase (ALP), and free fatty acid (FFA) secretion. In addition, transduced WT Tat-DJ-1 protein significantly inhibited the activation of NF-リB and MAPK (ERK and p38) expression as well as COX-2 and iNOS expression in STZ exposed pancreas. In contrast, there were no protective effects of treatment with C106A mutant Tat-DJ-1 protein. Collectively our results indicate that WT Tat-DJ-1 protein significantly ameliorates pancreatic tissues in STZ exposed diabetic mice.
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| Abstract, Accepted Manuscript(in press) [Submitted on May 2, 2018, Accepted on June 1, 2018] |
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