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High expression of RAD51 promotes DNA damage repair and survival in KRAS-mutant lung cancer cells
Zhiguo Zhang1, Lei Zhao2, Li Li1, Wei Zuo3, Lei Han1,*
1Department of Oncology, Beijing Daxing District People’s Hospital, Capital Medical University,
2Cancer center, Beijing Friendship Hospital, Capital Medical University,
3Department of respiration, First Affiliated Hospital of Nanchang University
Abstract
RAD51 recombinase plays a critical role in homologous recombination and DNA damage repair. Here we showed that expression of RAD51 is frequently upregulated in lung cancer tumors compared with normal tissues and is associated with poor survival (hazard ratio (HR) = 2, P = 0.0009). Systematic investigation of lung cancer cell lines revealed higher expression of RAD51 in KRAS mutant (MT) cells compared to wildtype (WT) cells. We further showed that MT KRAS, but not WT KRAS, played a critical role in RAD51 overexpression via MYC. Moreover, our results revealed that KRAS MT cells are highly dependent on RAD51 for survival and depletion of RAD51 resulted in enhanced DNA double strand breaks, defective colony formation and cell death. Together, our results suggest that mutant KRAS promotes RAD51 expression to enhance DNA damage repair and lung cancer cell survival, suggesting that RAD51 may be an effective therapeutic target to overcome chemo/radioresistance in KRAS mutant cancers.
Abstract, Accepted Manuscript(in press) [Submitted on September 14, 2018, Accepted on November 12, 2018]
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