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Protocadherin-7 contributes to maintenance of bone homeostasis through regulation of osteoclast multinucleation
Hyunsoo Kim1,# (Researcher), Noriko Takegahara1,# (Researcher), Matthew Walsh1 (Researcher), Jun Ueda2 (Researcher), Yoshitaka Fujihara2 (Researcher), Masahito Ikawa2 (Professor), Yongwon Choi 1,* (Professor)
1Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School,
2Research Institute for Microbial Diseases, Osaka University
Abstract
Osteoclasts are hematopoietic-derived cells that resorb bone. They are required to maintain proper bone homeostasis and skeletal strength. Although osteoclast differentiation depends on receptor activator of NF-リB ligand (RANKL) stimulation, additional molecules further contribute to osteoclast maturation. Here, we demonstrate that protocadherin-7 (Pcdh7) regulates formation of multinucleated osteoclasts and contributes to maintenance of bone homeostasis. We found that Pcdh7 expression is induced by RANKL stimulation, and that RNAi-mediated knockdown of Pcdh7 resulted in impaired formation of osteoclasts. We generated Pcdh7-deficient mice and found increased bone mass due to decreased bone resorption but without any defect in bone formation. Using an in vitro culture system, it was revealed that formation of multinucleated osteoclasts is impaired in Pcdh7-deficient cultures, while no apparent defects were observed in differentiation and function of Pcdh7-deficient osteoblasts. Taken together, these results reveal an osteoclast cell-intrinsic role for Pcdh7 in maintaining bone homeostasis.
Abstract, Accepted Manuscript(in press) [Submitted on March 6, 2020, Accepted on April 6, 2020]
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