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GDNF secreted by pre-osteoclasts induces migration of bone marrow mesenchymal stem cells and stimulates osteogenesis
Sol Yi1,2 (Graduate student), Jihee Kim1,2 (Graduate student), Soo Young Lee 1,2,* (Professor)
1Department of Life Science and 2The Research Center for Cellular Homeostasis, Ewha Womans University
Abstract
Bone resorption is linked to bone formation via temporal and spatial coupling within the remodeling cycle. Several lines of evidence point to the critical role of coupling factors derived from pre-osteoclasts (POCs) during the regulation of bone marrow-derived mesenchymal stem cells (BMMSCs). However, the role of glial cell-derived neurotrophic factor (GDNF) in BMMSCs is not completely understood. Herein, we demonstrate the role of POC-derived GDNF in regulating the migration and osteogenic differentiation of BMMSCs. RNA sequencing of POCs revealed that GDNF potentially stimulates BMMSC migration and osteogenic differentiation in vitro. Specifically, BMMSC migration was inhibited by a neutralizing antibody against GDNF in pre-osteoclast-conditioned medium (POC-CM), whereas treatment with a recombinant GDNF enhanced migration and osteogenic differentiation. In addition, POC-CM derived from GDNF knock-downed bone marrow macrophages suppressed BMMSC migration and osteogenic differentiation. SPP86, a small molecule inhibitor, inhibits BMMSC migration and osteogenic differentiation by targeting the receptor tyrosine kinase RET, which is recruited by GDNF into the GFR1 complex. Overall, this study highlights the function of POC-derived GDNF in BMMSC migration and osteogenic differentiation and suggests that GDNF is a potential therapeutic target in bone disorders.
Abstract, Accepted Manuscript(in press) [Submitted on September 17, 2020, Accepted on October 28, 2020]
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