Advances in higher-order chromatin architecture: The move towards 4D genome |
Namyoung Jung 1 (Postdoctoral fellow), Tae-Kyung Kim 1,2,* (Associate Professor) |
1Department of Life Sciences, Pohang University of Science and Technology (POSTECH), 2Yonsei University |
Abstract
In eukaryotes, the genome is hierarchically packed inside the nucleus, which facilitates physical contact between cis-regulatory elements (CREs), such as enhancers and promoters. Accumulating evidence highlights the critical role of higher-order chromatin structure in precise regulation of spatiotemporal gene expression under diverse biological contexts including lineage commitment and cell activation by external stimulus.
Genomics and imaging-based technologies, such as Hi-C and DNA fluorescence in situ hybridization (FISH), have revealed the key principles of genome folding, while newly developed tools focus on improvement in resolution, throughput and modality at single-cell and population levels, and challenge the knowledge obtained through conventional approaches. In this review, we discuss recent advances in our understanding of principles of higher-order chromosome conformation and technologies to investigate 4D chromatin interactions.
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Abstract, Accepted Manuscript [Submitted on March 4, 2021, Accepted on April 12, 2021] |
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