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This galley proof is being listed electronically before publishing the final manuscript (It's not final version).

 
Visualization of chromatin higher-order structures and dynamics in live cells
Tae Lim Park1 (Graduate student), YigJi Lee1 (Graduate student), Won-Ki Cho 1,2,* (Professor)
1Department of biological sciences and 2KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST), 291 Deahak-ro, Yuseong-gu, Daejeon, 34141, South Korea
Abstract
Chromatin has highly organized structures in the nucleus, and these higher-order structures are proposed to regulate gene activities and cellular processes. Sequencing-based techniques, such as Hi–C, and fluorescent in situ hybridization (FISH) have revealed a spatial segregation of active and inactive compartments of chromatin, as well as the non-random positioning of chromosomes in the nucleus, respectively. However, regardless of their efficiency in capturing target genomic sites, these techniques are limited to fixed cells. Since chromatin has dynamic structures, live cell imaging techniques are highlighted for their ability to detect conformational changes in chromatin at a specific time point, or to track various arrangements of chromatin through long-term imaging. Given that the imaging approaches to study live cells are dramatically advanced, we recapitulate methods that are widely used to visualize the dynamics of higher-order chromatin structures.
Abstract, Accepted Manuscript [Submitted on July 22, 2021, Accepted on August 29, 2021]
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