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This galley proof is being listed electronically before publishing the final manuscript (It's not final version).

 
Gold and Silver Plasmonic Nanoprobes Trace the Positions of Histone Codes
Inhee Choi 1 (Professor), Jihwan Song 2 (Professor), Hyunsung Park 1,* (Professor)
1Department of Life Science, University of Seoul, Seoul 02504, Republic of Korea,
2Department of Mechanical Engineering, Hanbat National University, Daejeon 34158, Republic of Korea
Abstract
We visualized the distribution of heterochromatin in a single nucleus using plasmonic nanoparticle-conjugated H3K9me3 and H3K27me3 antibodies. Due to distance-dependent plasmonic coupling effects between nanoprobes, their scattering spectra shift to longer wavelengths as the distance between heterochromatin histone markers reduced during oncogene-induced senescence (OIS). These observations were supported by simulating scattering profiles based on considerations of particle numbers, interparticle distances, and the spatial arrangements of plasmonic nanoprobes. Using this plasmon-based colourimetric imaging, we estimated changes in distances between H3K9me3 and H3K27me3 during the formation of senescence-associated heterochromatin foci in OIS cells. We anticipate that the devised analytical technique combined with high-spatial imaging and spectral simulation will eventually lead to a new means of diagnosing and monitoring disease progression and cellular senescence.
Abstract, Accepted Manuscript [Submitted on January 6, 2022, Accepted on January 6, 2022]
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