Gold and Silver Plasmonic Nanoprobes Trace the Positions of Histone Codes |
Inhee Choi 1 (Professor), Jihwan Song 2 (Professor), Hyunsung Park 1,* (Professor) |
1Department of Life Science, University of Seoul, Seoul 02504, Republic of Korea, 2Department of Mechanical Engineering, Hanbat National University, Daejeon 34158, Republic of Korea |
Abstract
We visualized the distribution of heterochromatin in a single nucleus using plasmonic nanoparticle-conjugated H3K9me3 and H3K27me3 antibodies. Due to distance-dependent plasmonic coupling effects between nanoprobes, their scattering spectra shift to longer wavelengths as the distance between heterochromatin histone markers reduced during oncogene-induced senescence (OIS). These observations were supported by simulating scattering profiles based on considerations of particle numbers, interparticle distances, and the spatial arrangements of plasmonic nanoprobes. Using this plasmon-based colourimetric imaging, we estimated changes in distances between H3K9me3 and H3K27me3 during the formation of senescence-associated heterochromatin foci in OIS cells. We anticipate that the devised analytical technique combined with high-spatial imaging and spectral simulation will eventually lead to a new means of diagnosing and monitoring disease progression and cellular senescence.
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Abstract, Accepted Manuscript [Submitted on January 6, 2022, Accepted on January 6, 2022] |
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