2-Undecanone derived from Pseudomonas aeruginosa modulates neutrophil activity |
Yu Sun Jeong1 (Graduate student), Sunghyun Huh2,3 (Graduate student), Ji Cheol Kim1 (Graduate student), Ji Ye Park1 (Graduate student), ChaeEun Lee1 (Graduate student), Min-Sik Kim1 (Professor), JaeHyung Koo 1 (Professor), Yoe-Sik Bae 1,* (Professor) |
1Department of Biological Sciences, Sungkyunkwan University, 2Department of New Biology and 3New Biology Research Center (NBRC), DGIST |
Abstract
Pseudomonas aeruginosa (P. aeruginosa) is a well-known Gram-negative opportunistic pathogen. Neutrophils play key roles for host defense against P. aeruginosa infection. In this study, we identified a metabolite derived from P. aeruginosa regulates neutrophil activities. Using gas chromatography-mass spectrometry, we found that 2-undecanone is markedly increased in the peritoneal fluid of P. aeruginosa-infected mice. 2-Undecanone elicited the activation of neutrophils in a Gメi-phospholipase C pathway. However, 2-undecanone strongly inhibited responses to lipopolysaccharide and bactericidal activity of neutrophils against P. aeruginosa by inducing apoptosis. Our results demonstrate that 2-undecanone from P. aeruginosa limits the innate defense activity of neutrophils, suggesting that production of inhibitory metabolites is a strategy of P. aeruginosa for escaping the host immune system.
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Abstract, Accepted Manuscript(in press) [Submitted on January 10, 2022, Accepted on April 5, 2022] |
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