Over the past few years, hydrogen sulfide (H2S) has been known to exert several biological functions in mammalian. The production of H2S is mainly produced by cystathione β-synthase (CBS), 3-mercaptopyruvate sulfur transferase (3-MST), and cystathione γ-lyase (CSE). They are broadly induced in liver and involved in liver function by working on a variety of molecular targets. As an important regulator of liver function, H2S regulates the pathogenesis of several liver diseases, such as non-alcoholic steatohepatitis (NASH) and liver fibrosis. Targeting H2S-generating enzymes is possible to be a therapeutic target for controlling liver diseases. This review described the function of H2S in liver disease and summarized recent characterized role of H2S in several cellular process of the liver.