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Preventive effects of nano-graphene oxide against Parkinson's disease via reactive oxygen species scavenging and anti-inflammation
Hyung Ho Yoon 1,*,# (Researcher), Hee-Yeong Kim 2,# (Graduate student), Hanyu Seong 3,# (Professor), Dong Kwang Seo 1 (Clinical fellow), Soon Won Choi 2,4 (CTO), Kyung-Sun Kang 2 (Professor), Sang Ryong Jeon 1 (Professor)
1Department of Neurological Surgery, Asan Medical Center,
2Adult Stem Cell Research Center and Research Institute for Veterinary Science, Seoul National University,
3Department of Neurosurgery, Seoul Bumin Hospital,
4Institute of Bio & Nano Convergence, Biogo Co.
We investigated the neuroprotective effects of deca nano-graphene oxide (daNGO) against reactive oxygen species (ROS) and inflammation in the human neuroblastoma cell line SH-SY5Y and in the 6-hydroxydopamine (6-OHDA) induced Parkinsonian rat model. An MTT assay was performed to measure cell viability in vitro in the presence of 6-OHDA and/or daNGO. The intracellular ROS level was quantified using 2∏,7∏-dichlorofluorescein diacetate. daNGO showed neuroprotective effects against 6-OHDA-induced toxicity and also displayed ROS scavenging properties. We then tested the protective effects of daNGO against 6-OHDA induced toxicity in a rat model. Stepping tests showed that the akinesia symptoms were improved in the daNGO group compared to the control group. Moreover, in an apomorphine-induced rotation test, the number of net contralateral rotations was decreased in the daNGO group compared to the control group. By immunofluorescent staining, the animals in the daNGO group had more tyrosine hydroxylase-positive cells than the controls. By anti-Iba1 staining, 6-OHDA induced microglial activation showed a significantly decrease in the daNGO group, indicating that the neuroprotective effects of graphene resulted from anti-inflammation. In conclusion, nano-graphene oxide has neuroprotective effects against the neurotoxin induced by 6-OHDA on dopaminergic neurons.
Abstract, Accepted Manuscript(in press) [Submitted on September 8, 2022, Accepted on November 23, 2022]
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