BMB Reports Papers in Press available online.

Search Papers In Press
This galley proof is being listed electronically before publishing the final manuscript (It's not final version).

Cytosolic domain regulates the calcium sensitivity and surface expression of BEST1 channels in the HEK293 cells
Kwon Woo Kim1,# (researcher), Junmo Hwang1,# (researcher), Dong-Hyun Kim1 (researcher), Hyungju Park1 (principal researcher), Hyun-Ho Lim 1,2,* (principal researcher)
1Neurovascular Research Group, Korea Brain Research Institute (KBRI),
2Department of Brain Sciences, Daegu Gyeongbuk Institute of Science & Technology (DGIST)
BEST family is a class of Ca2+-activated Cl- channels evolutionary well conserved from bacteria to human. The human BEST paralogs (BEST1 – BEST4) share significant amino acid sequence homology in the N-terminal region, which forms the transmembrane helicases and contains the direct calcium-binding site, Ca2+-clasp. But the cytosolic C-terminal region is less conserved in the paralogs. Interestingly, this domain-specific sequence conservation is also found in the BEST1 orthologs. However, the functional role of the C-terminal region in the BEST channels is still poorly understood. Thus, we aimed to understand the functional role of the C-terminal region in the human and mouse BEST1 channels by using electrophysiological recordings. We found that the calcium-dependent activation of BEST1 channels can be modulated by the C-terminal region. The C-terminal deletion hBEST1 reduced the Ca2+-dependent current activation and the hBEST1-mBEST1 chimera showed a significantly reduced calcium sensitivity to hBEST1 in the HEK293 cells. And the C-terminal domain could regulate cellular expression and plasma membrane targeting of BEST1 channels. Our results can provide a basis for understanding the C-terminal roles in the structure-function of BEST family proteins.
Abstract, Accepted Manuscript(in press) [Submitted on October 24, 2022, Accepted on January 2, 2023]
  © KSBMB. All rights reserved. / Powered by INFOrang Co., Ltd