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Rice CHD3/Mi-2 chromatin remodeling factor RFS regulates vascular development and root formation by modulating transcription of auxin-related genes NAL1 and OsPIN1
Hyeryung Yoon1 (Postdoc), Chaemyeong Lim1 (Postdoc), Bo Lyu2 (Graduate student), Qisheng Song2 (Professor), Soyon Park2 (Professor), Kiyoon Kang3 (Professor), Sung-Hwan Cho1 (Professor), Nam-Chon Paek 1,* (Professor)
1Department of Agriculture, Forestry and Bioresources, Seoul National University,
2Division of Plant Science and Technology, University of Missouri,
3Division of Life Sciences, Incheon National University
Abstract
Vascular system in plants facilitates the long-distance transportation of water and nutrients through the xylem and phloem, while also providing mechanical support for vertical growth. Although many genes that regulate vascular development in rice have been identified, the mechanism by which epigenetic regulators control vascular development remains unclear. This study found that Rolled Fine Striped (RFS), a Chromodomain Helicase DNA-binding 3 (CHD3)/Mi-2 subfamily protein, regulates vascular development in rice by affecting the initiation and development of primordia. The rfs mutant was found to affect auxin-related genes, as revealed by RNA sequencing and reverse transcription-quantitative PCR analysis. The transcript levels of OsPIN1 and NAL1 genes were downregulated in rfs mutant compared to wild-type plant. The chromatin immunoprecipitation assays showed that OsPIN1a and NAL1 genes had lower levels of H3K4me3 in rfs mutant. Furthermore, exogenous auxin treatment partially rescued the reduced adventitious root vascular development in rfs mutant. Subsequently, exogenous treatments with auxin or an auxin-transport inhibitor revealed that the expression of OsPIN1a and NAL1 is mainly affected by auxin. These results provide strong evidence that RFS plays an important role in vascular development and root formation through the auxin signaling pathway in rice.
Abstract, Accepted Manuscript [Submitted on January 13, 2024, Accepted on February 23, 2024]
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