Identification of optimal adenine and cytosine base editors for genome editing in Arabidopsis and soybean |
Yeong Yeop Jeong1,3,# (Graduate Student), Jun Hee Han4,# (Graduate Student), Jihyeon Yu5 (Post-doc), Sangsu Bae 5,6,7 (Associate Professor), Pil Joon Seo 1,2,3,* (Associate Professor) |
1Plant Genomics and Breeding Institute and 2Department of Chemistry, Seoul National University, 3Department of Biological Sciences, Sungkyunkwan University, 4Department of Chemistry, Hanyang University, 5Medical Research Center of Genomic Medicine Institute and 6Cancer Research Institute, Seoul National University, 7Department of Biomedical Sciences, Seoul National University College of Medicine |
Abstract
Base editors including adenine base editors (ABEs) and cytosine base editors (CBEs) are used widely in many organisms to introduce site-specific sequence modifications in genomic DNA without causing double-strand breaks (DSBs). However, base editors have low editing efficiencies especially in dicot plants, which limits their use in dicot plant genome engineering. Here, we evaluated the editing efficiencies of various base editors to determine which ones are optimal for base editing in dicot plants. We found that ABE8e, an ABE variant, showed superior A-to-G base editing efficiency within A5-A8 windows, and A3A/Y130F-V04, a CBE variant, showed the highest C-to-T base editing efficiency within C4-C15 windows in both Arabidopsis and soybean protoplasts. Overall, we propose that these two base editors could be used as first choices for efficient genome engineering in a variety of crop plants.
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Abstract, Accepted Manuscript [Submitted on June 28, 2024, Accepted on October 11, 2024] |
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