Arginine methylation, which is catalyzed by protein arginine methyltransferases (Prmts), is known to play a key regulator role in various biological processes. However, the function of Prmts in osteogenic differentiation of mesenchymal stem cells (MSCs) has not been clearly understood. In the current study, we attempt to elucidate a positive role of Prmt7 in osteogenic differentiation. Prmt7-depleted C3H/10T1/2 or bone marrow mesenchymal stem cells (BMSCs) showed the attenuation in the expression of osteogenic specific genes and Alizarin red staining compared to the wild type cells. Furthermore, we found that Prmt7 deficiency caused the reduction in the activation of BMP signaling cascade, which is essential for the regulation of cell fate commitment and osteogenesis. Taken together, our data indicate that Prmt7 plays important roles as a critical regulator of osteogenic differentiation.